The effects of fructose-1,6-bisphosphate and dexamethasone on acute inflammation and T-cell proliferation.

OBJECTIVE AND DESIGN: Chronic glucocorticoid treatment is associated with pharmacological resistance. We investigated the auxiliary effects of fructose-1,6-bisphosphate (FBP) on dexamethasone (DEX)-related modulation of inflammation and T-cell proliferation. METHODS: Acute inflammation (pleurisy) was induced by injection of carrageenan into the pleural cavity of rats that were treated in vivo with DEX s. c. and FBP i. p. Peripheral blood mononuclear cells were isolated and T-cell sensitivity to FBP and DEX was evaluated in vitro. RESULTS: FBP and DEX reduced the exudate volume, protein concentration and neutrophils in the pleural cavity. However no synergistic effects were observed when these compounds were tested simultaneously. In contrast, both compounds dose-dependently and synergistically suppressed T-cell proliferation. CONCLUSION: These data suggest that FBP may be beneficial as auxiliary drug for the treatment of patients with acquired glucocorticoid resistance.

Effect of fructose-1,6-bisphosphate in the cold storage solution after 12 and 36 hours of rat liver preservation.

Fructose-1,6-bisphosphate (FBP) has been reported to have a protective effect on liver injury following ischemic/reperfusion periods because it maintains ATP levels during cold preservation. In the present study, we evaluated the effects of addition of FBP to storage solutions for cold liver preservation during 12 or 36 hours. Adult male Wistar rats were randomly divided into three experimental groups. The hepatic perfusion and preservation were performed with these solutions: UW; UW plus 10 mmol/L FBP; and FBP 10 mmol/L (FBPS) alone. The biochemical measurements of AST and ALT were performed on samples of the cold storage solution after 12- or 36-hour preservation. UW and FBPS solutions showed similar preservation grades at 12 hours. Addition of 10 mmol/L of FBP to UW solution induced liver injury and a poor preservation grade during 12 or 36 hours. UW solution was better than FBPS after 36 hours preservation. UW solution continues to offer a superior performance for liver preservation during long times; however, FBPS may be an alternative for short cold preservation times.

Protective effect of fructose-1,6-bisphosphate in the cold storage solution for liver preservation in rat hepatic transplantation.

Fructose-1,6-bisphosphate (FBP) has been reported to have a protective effect on liver injury following ischemic/reperfusion periods. FBP maintains ATP levels and thereby cellular energy metabolism, which is important to the liver during cold preservation. In the present study, we evaluated the effects of FBP on the composition of storage solutions for cold liver preservation. Adult male Wistar rats were randomly divided into three experimental groups. Hepatic perfusion and preservation were performed with UW, UW plus 10 mmol/L FBP (UWM), and FBP 10 mmol/L (FBPS) alone solutions. Biochemical measurements of AST, ALT, and TBARS were performed on samples of the cold storage solution at 0, 12, 18, and 24 hours preservation. FBPS and UW solutions showed similar preservation grades during 18 hours. Addition of 10 mmol/L of FBP to UW solution induced liver injury and a poor preservation grade. FBP appears to protect the liver from injury caused by free radicals when the preservation time is less than 18 hours. Therefore, FBP may exert a protective effect for the preservation of livers during cold storage, and could represent an important component of new cold storage solutions.

Sepse relacionada ao uso de cateter intravascular / Intravascular catheter - related sepsis

O objetivo deste trabalho foi relacionar de forma retrospectiva, o uso de cateteres intravasculares em pacientes hospitalizados em Unidades de Tratamento Intensivo (UTIs) com a incidência de bacteremia e sepse. Os cateteres (n = 250) foram semeados em ágar suplementado com 5% de sangue de carneiro. A cultura foi semi-quantitativa e, considerada positiva, quando encontrou-se o crescimento de 15 ou mais colônias em placa de agar. As hemoculturas foram incubadas e monitoradas pelo sistema Bact Alert (Organon Teknika). A identificação microbiana foi realizada através de provas convencionais ou sistemas automatizados. Do total de 250 cateteres, 12,4% tiveram cultura positiva (31/250), com maior frequência para Staphylococcus sp. coagulase negativa (49%). Foram realizadas hemoculturas de 13 pacientes que tiveram cultura positiva para o cateter intracath, sendo que 7 (58,8%) foram positivas e 4 dessas hemoculturas apresentaram o mesmo microorganismo encontrado no cateter, sinalizando uma bacteremia devida ao cateter (30,7%). Dos 7 pacientes, 4 (57%) evoluíram clinicamente para sepse e chegaram ao óbito. Esse achado, demonstra uma relação importante entre a implantação de cateteres intravasculares e o desenvolvimento de bacteremia e sepse.

The aim of this paper was to analyze a retrospective way from the use of catheters in patient hospitalized in Intensive Care Units, with bacteraemia incidence and sepsis. The catheters (n=250) were sowed in agar blood (5% of sheep blood). The culture was semi-quantitative and considered positive, when were found the growth 15 or more colonies in agar plate...